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The solutions of Hampton Research's Crystal Screen 2 have been reordered for compatibility
with Douglas Instruments ASPRUN software. There are three sections: viscous organic
precipitants, non-viscous organic precipitants and inorganic precipitants. Within each
section solutions are ordered alphabetically by precipitant, then by increasing
concentrations, and then by pH where a precipitant is used at the same concentration more
than once. The inorganic precipitants are ordered by anion followed by cation.
To avoid confusion, solutions should always be referred to by letter and number,
e.g. Q1 or J18.
| No. |
Precipitant |
Buffer |
Additive |
Hampton No. |
| Q1 |
10% Jeffamine M-600 |
Na Citrate pH 5.6 |
0.01 M Fe Chloride |
J18 |
| Q2 |
20% Jeffamine M-600 |
Hepes pH 7.5 |
None |
J31 |
| Q3 |
30% Jeffamine M-600 |
MES pH 6.5 |
0.05 M Cs Chloride |
J24 |
| Q4 |
30% MPD |
Na Acetate pH 4.6 |
0.2 M Na Chloride |
J10 |
| Q5 |
30% MPD |
Hepes pH 7.5 |
0.5 M NH4 Sulfate |
J29 |
| Q6 |
50% MPD |
Tris pH 8.5 |
0.2 M NH4 Phosphate |
J43 |
| Q7 |
70% MPD |
Hepes pH 7.5 |
None |
J35 |
| Q8 |
30% PEG 400 |
Na Acetate pH 4.6 |
0.1 M Cd Chloride |
J12 |
| Q9 |
25% PEG MME 550 |
MES pH 6.5 |
0.01 M Zn Sulfate |
J27 |
| Q10 |
30% PEG MME 550 |
Bicine pH 9.0 |
0.1 M Na Chloride |
J46 |
| Q11 |
20% PEG MME 2000 |
Tris pH 8.5 |
0.01 M Ni Chloride |
J45 |
| Q12 |
30% PEG MME 2000 |
Na Acetate pH 4.6 |
0.2 M NH4 Sulfate |
J13 |
| Q13 |
30% PEG MME 5000 |
MES pH 6.5 |
0.2 M NH4 Sulfate |
J26 |
| Q14 |
10% PEG 6000 |
None |
2.0 M Na Chloride |
J1 |
| Q15 |
10% PEG 6000
5% MPD |
Hepes pH 7.5 |
None |
J30 |
| Q16 |
10% PEG 8000
10% PEG 1000 |
None |
None |
J7 |
| Q17 |
10% PEG 8000
8% Ethylene Glycol |
Hepes pH 7.5 |
None |
J37 |
| Q18 |
20% PEG 10000 |
Hepes pH 7.5 |
None |
J38 |
| Q19 |
10% PEG 20000 |
Bicine pH 9.0 |
2% Dioxane |
J48 |
| Q20 |
12% PEG 20000 |
MES pH 6.5 |
None |
J22 |
| Q21 |
4% Polyethyleneimine |
Na Citrate pH 5.6 |
0.5 M Na Chloride |
J16 |
| Q22 |
25% tert-Butanol |
Tris pH 8.5 |
0.1 M Ca Chloride |
J40 |
| Q23 |
35% tert-Butanol |
Na Citrate pH 5.6 |
None |
J17 |
| Q24 |
35% Dioxane |
None |
None |
J4 |
| Q25 |
10% Ethanol |
None |
1.5 M Na Chloride |
J8 |
| Q26 |
20% Ethanol |
Tris pH 8.5 |
None |
J44 |
| Q27 |
25% Ethylene glycol |
None |
None |
J3 |
| Q28 |
12% Glycerol |
Tris pH 8.5 |
1.5 M NH4 Sulfate |
J42 |
| Q29 |
1.0 M 1,6 Hexanediol |
Na Acetate pH 4.6 |
0.01 M Co Chloride |
J11 |
| Q30 |
2.5 M 1,6 Hexanediol |
Na Citrate pH 5.6 |
0.01 M Mn Chloride |
J19 |
| Q31 |
3.4 M 1,6 Hexanediol |
Tris pH 8.5 |
0.2 M Mg Chloride |
J39 |
| Q32 |
1.0 M Imidazole pH 7.0 |
None |
None |
J6 |
| Q33 |
1.0 M Na Acetate |
Hepes pH 7.5 |
0.05 M Cd Sulfate |
J34 |
| Q34 |
2.0 M Mg Chloride |
Bicine pH 9.0 |
None |
J47 |
| Q35 |
0.5 M Na Chloride
0.01 M Mg Chloride |
None |
0.01 M Cetyl trimethyl ammonium bromide |
J2 |
| Q36 |
2.0 M Na Chloride |
Na Acetate pH 4.6 |
None |
J9 |
| Q37 |
2.0 M Na Chloride |
MES pH 6.5 |
0.2 M K,Na Phosphate |
J21 |
| Q38 |
4.3 M Na Chloride |
Hepes pH 7.5 |
None |
J36 |
| Q39 |
1.6 M Na Citrate pH 6.5 |
None |
None |
J28 |
| Q40 |
1.0 M Li Sulfate |
Na Citrate pH 5.6 |
0.5 M NH4 Sulfate |
J15 |
| Q41 |
1.0 M Li Sulfate |
Tris pH 8.5 |
0.01 M Ni Chloride |
J41 |
| Q42 |
1.6 M Mg Sulfate |
MES pH 6.5 |
None |
J20 |
| Q43 |
1.6 M NH4 Sulfate |
MES pH 6.5 |
10% Dioxane |
J23 |
| Q44 |
1.6 M NH4 Sulfate |
Hepes pH 7.5 |
0.1 M Na Chloride |
J32 |
| Q45 |
1.8 M NH4 Sulfate |
MES pH 6.5 |
0.01 M Co Chloride |
J25 |
| Q46 |
2.0 M NH4 Sulfate |
None |
5% Isopropanol |
J5 |
| Q47 |
2.0 M NH4 Sulfate |
Na Citrate pH 5.6 |
0.2 M K,Na Tartrate |
J14 |
| Q48 |
2.0 M NH4 Sulfate |
Hepes pH 7.5 |
None |
J33 |
| Hampton No. |
Ordered by Precipitant |
| J1 |
Q14 |
| J2 |
Q35 |
| J3 |
Q27 |
| J4 |
Q24 |
| J5 |
Q46 |
| J6 |
Q32 |
| J7 |
Q16 |
| J8 |
Q25 |
| J9 |
Q36 |
| J10 |
Q4 |
| J11 |
Q29 |
| J12 |
Q8 |
| J13 |
Q12 |
| J14 |
Q47 |
| J15 |
Q40 |
| J16 |
Q21 |
| J17 |
Q23 |
| J18 |
Q1 |
| J19 |
Q30 |
| J20 |
Q42 |
| J21 |
Q37 |
| J22 |
Q20 |
| J23 |
Q43 |
| J24 |
Q3 |
| J25 |
Q45 |
| J26 |
Q13 |
| J27 |
Q9 |
| J28 |
Q39 |
| J29 |
Q5 |
| J30 |
Q15 |
| J31 |
Q2 |
| J32 |
Q44 |
| J33 |
Q48 |
| J34 |
Q33 |
| J35 |
Q7 |
| J36 |
Q38 |
| J37 |
Q17 |
| J38 |
Q18 |
| J39 |
Q31 |
| J40 |
Q22 |
| J41 |
Q41 |
| J42 |
Q28 |
| J43 |
Q6 |
| J44 |
Q26 |
| J45 |
Q11 |
| J46 |
Q10 |
| J47 |
Q34 |
| J48 |
Q19 |
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