General |
| Crystallization Methods |
1. Microbatch |
|
2. Sitting Drop |
| Ambient temperature |
4 to 30°C |
| Computer requirement |
PC with Windows XP or Windows Vista |
Microbatch Method
|
| Volume range of crystallization trial |
0.2 to 10 microliters |
| Typical volume of crystallization trial for
screening. |
0.4 microliters |
| Typical volume of crystallization trial for
optimization |
2 microliters (Oryx8 only) |
| Volume of protein dispensed per trial |
0.1 to 5 microliters |
| Number of crystallization trials set up per run |
1 to 192 (or more) |
| Number of solutions dispensed in each trial for optimization |
1 to 7 (including water) |
| Plates accomodated |
All high quality plates can be used |
Vapor Diffusion Method
|
| Volume of droplet |
0.2 to 10 microliters |
| Plates accommodated |
All high quality plates can be used |
| Number of wells dispensed |
1 to 96x3 |
Microtips
|
| Number of bores |
7, 5, 4, 3 or 2 |
| Cross-section of microtip at tip |
0.45 - 0.95 mm |
| Cross-section of each bore |
0.15 mm I.D. |
| Dead volume |
Zero |
| Material |
Water repellent fluoropolymer. Protein
channel is treated to prevent the air-bubble from breaking up. |
Universal Syringe Driver
|
| Number of discrete steps for syringe volume |
More than 22,000 |
| Displacement for each step |
3.2 microns |
| Volume dispensed per step with a 100 microlitre syringe. |
5.2 nl |
| Maximum speed |
4.0 mm/second |
Accuracy of Universal Syringe
Driver |
| R.M.S. error per step |
+/- 8% |
| Nominal maximum error per step |
16% |
| Nominal maximum cumulative error over complete linear
displacement |
2 steps |
Automatic XYZV Plate Loader
|
| Linear displacement of table: |
| travel, first horizontal axis (X) |
321 mm |
| travel, second horizontal axis (Y) |
151 mm |
| travel, first vertical axis (Z) |
50 mm |
| travel, second vertical axis (V) |
52 mm |
| Nominal maximum cumulative error over complete linear
displacement |
0.1 mm |
| Length required on bench |
670 mm |
| Depth required on bench |
320 mm |
| Height in extended position |
220 mm |
| Weight |
11.8 kg |
| Time taken to dispense 96 well experiment into e.g Douglas Vapor Batch Plate |
15 min |
| Maximum speed: |
| X |
75 mm/s |
| Y |
55 mm/s |
| Z &V |
25 mm/s |
| The speed is software controllable and is fully
variable |
