 |
|
|
|
|
|
|
Differences
Between Techniques
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| • |
Amount of Protein
|
|
|
|
– |
Hanging drop,
sitting drop, and capillary techniques use copious
|
|
amounts of
protein and crystallizing agent
|
|
|
|
– |
Microbatch
diffusion uses 300nl of protein per condition (in this
|
|
|
experiment)
|
|
|
|
– |
Microbatch allows
use of same mother liquor for many
|
|
|
experiments
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| • |
Time
|
|
|
|
– |
Hanging drop,
sitting drop, and capillary methods require a great
|
|
deal of time
because solutions must be degassed
|
|
|
|
– |
Douglas robots
only need initial set up and then the robot
|
|
|
completes the
experiment
|
|
|
|
|