References

New methods and theoretical studies:

Patrick D. Shaw Stewart, Stefan A. Kolek, Richard A. Briggs, Naomi E. Chayen and Peter F.M. Baldock. 'Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization'. Cryst. Growth Des., 2011, 11 (8), pp 3432–3441. On-line at http://pubs.acs.org/doi/abs/10.1021/cg2001442. If you do not have a subscription to Crystal Growth and Design, click here to obtain a free copy.

Jose A. Gavira, M. A. Hernandez-Hernandez, Luis A. Gonzalez-Ramirez, Richard A. Briggs, Stefan A. Kolek, and Patrick D. Shaw Stewart. "Combining Counter-Diffusion and Microseeding to Increase the Success Rate in Protein Crystallization." Cryst. Growth Des., 2011, 11 (6), pp 2122–2126. Available on-line at http://pubs.acs.org/doi/abs/10.1021/cg101380j

Garcia-Caballero, Shaw Stewart, Briggs, Kolek and others. "Optimization of Protein Crystallization: The OptiCryst Project". Cryst. Growth Des., 2011, 11 (6), pp 2112–2121. Available on-line at http://pubs.acs.org/doi/abs/10.1021/cg1013768

Galina Obmolova,* Thomas J. Malia, Alexey Teplyakov, Raymond Sweet and Gary L. Gilliland. 'Promoting crystallization of antibody–antigen complexes via microseed matrix screening.' Acta Crystallographica Section D 66 (2010) 927–933. Open-access at http://journals.iucr.org/d/issues/2010/08/00/bw5361/bw5361.pdf

A. G. Villaseñor, A. Wong, A. Shao, A. Garg, A. Kuglstatter and S. F. Harris. 'Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias.' Acta Crystallographica Section D 66 (2010) 568-576. On-line at http://scripts.iucr.org/cgi-bin/paper?S0907444910005512

Allan D’Arcy, Frederic Villarda, May Marsh. An automated microseed matrix-screening method for protein crystallization. Acta Crystallographica section D 63 (2007), 550–554. (This paper describes the use of microseeding in the SCREENING step of crystallization - which has proved to be a very easy and powerful technique.)

Gregory Ireton and Barry Stoddard. 'Microseed matrix screening to improve crystals of yeast cytosine deaminase'. Acta Crystallographica section D60 (2004) 601–605. Available on-line at http://scripts.iucr.org/cgi-bin/paper?S0907444903029664

G. B. Mortuza, L.F. Haire, A. Stevens, S.J. Smerdon, J.P. Stoye, I.A. Taylor. High-resolution structure of a retroviral capsid hexameric amino-terminal domain. Nature 431 (2004), pp 481-485. (This paper describes the use of the vapor batch plate with isopropanol.)

A. D'Arcy, A. Mac Sweeney, M. Stihle, A. Haber. The advantages of using a modified microbatch method for rapid screening of protein crystallization conditions. Acta Crystallographica section D 59 (2003), pp 396-399.

P.F.M. Baldock, P.D. Shaw Stewart. Practical experimental design techniques for automatic and manual protein crystallization. Journal of Crystal Growth 196 (1999) 665 - 673

N.E. Chayen. Comparative studies of protein crystallization by vapor diffusion and microbatch. Acta Cryst. D54 (1998), pp 8 - 15. (This paper describes converting microbatch conditions to vapor diffusion, and also seems to be the first publication that mentions crystallization in less than 1 microlitre drops.)

P.F.M. Baldock, V. Mills, P.D. Shaw Stewart. A comparison of microbatch and vapour diffusion for initial screening of crystallization conditions. Journal of Crystal Growth. 168 (1996), pp 170-174.

A.D'Arcy, C.Elmore, M.Stihle, J.E.Johnston. A novel approach to crystallising proteins under oil. Journal of Crystal Growth 168 (1996) 175-180.

P.D. Shaw Stewart, M. Khimasia. Predispensed Gradient Matrices - a New Rapid Method of Finding Crystallization Conditions. Acta Crystallographica. D 50 (1994), pp 441-442.

E.E.G. Saridakis, P.D. Shaw Stewart, L.F. Lloyd, D.M. Blow. Phase Diagram and Dilution Experiments in the Crystallization of Carboxypeptidase G2. Acta Crystallographica. D 50 (1994), pp 293-297.

D.M. Blow, N.E. Chayen, L.F. Lloyd, E. Saridakis. Control of Nucleation of Protein Crystals. Protein Science. 3 (1994), pp 1638-1643.

N.E. Chayen, J. Radcliffe, D.M. Blow. The Control of Nucleation in the Crystallization of Lysozyme. Protein Science. 2 (1993), pp 113-118.

L.F.Lloyd, P.Brick, D.M.Blow. Crystallization studies on HIV-1 reverse transcriptase. Journal of Crystal Growth. 122 (1992), pp 355-359.

Description of Oryx and IMPAX (the predecessor of Oryx):

A.K. Shah, Zhi-Jie Liu, P.D. Shaw Stewart, F.D. Schubot, J.P. Rose, M.G. Newton and B-C. Wang. On increasing protein-crystallization throughput for X-ray diffraction studies. Acta Crystallographica D 61 (2005), pp 123-129

N.E. Chayen, P.D. Shaw Stewart, P. Baldock. New Developments of the IMPAX Small-Volume Automated Crystallization System. Acta Crystallographica. D 50 (1994), pp 456-458.

N.E. Chayen, P.D. Shaw Stewart, D.M. Blow. Microbatch Crystallization Under Oil - a New Technique Allowing Many Small-volume Crystallization Trials. Journal of Crystal Growth. 122 (1992), pp 176-180.

N.E. Chayen, P.D. Shaw Stewart, D.L. Maeder, and D.M. Blow. An Automated System for Micro-batch Protein Crystallization and Screening. Journal of Applied Crystallography. 23 (1990), pp 297-302.

Successful crystallizations using microbatch and Oryx:

S. Yamada, Y. Suzuki, T. Suzuki, M. Q. Le, C. A. Nidom, Y. Sakai-Tagawa, Y. Muramoto, M. Ito, M. Kiso, T. Horimoto, K. Shinya, T. Sawada, M. Kiso, T. Usui, T. Murata, Y. Lin, A. Hay, L. F. Haire, D. J. Stevens, R. J. Russell, S. J. Gamblin, J. J. Skehel & Y. Kawaoka. Haemagglutinin mutations responsible for the binding of H5N1 influenza A viruses to human-type receptors. Nature (444) 16 November 2006 doi:10.1038/nature05264

R. J. Russell, L. F. Haire, D. J. Stevens, P. J. Collins, Y. P. Lin, G. M. Blackburn, A. J. Hay, S. J. Gamblin & J. J. Skehel. The structure of H5N1 avian influenza neuraminidase suggests new opportunities for drug design. Nature, (443) Sept 7, 2006 45-49 (N1 neuraminidase structure solved with only 0.6 mg of protein using Oryx8).

The structure of H5N1 avian influenza neuraminidase suggests new opportunities for drug design R. J. Russell, L. F. Haire, D. J. Stevens, P. J. Collins, Y. P. Lin, G. M. Blackburn, A. J. Hay, S. J. Gamblin & J. J. Skehel. Nature, (443) Sept 7, 2006 45-49

Y. Korkhin, F. Frolow, O. Bogin, M. Peretz, A.J. Kalb, Y. Burstein. Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry. Acta Crystallographica. D 52 (1996), pp 882-886.

N.E. Chayen, E. Conti, C. Vieille, J.G. Zeikus. Crystallization and initial x-ray analysis of xylose isomerase from Thermotoga neapolitana. Acta Crystallographica. D 53 (1997), 2, pp 229-230.

F. Sica, S. Adinolfi, L. Vitagliano, A. Zagari, S. Capasso, L. Mazzarella. Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions. Journal of Crystal Growth. 168 (1996), 1-4, pp 192-197.

Y. Korkhin, F. Frolow, O. Bogin, M. Peretz, A.J. Kalb (Gilboa), Y. Burstein. Crystalline alcohol dehydrogenases from the mesophilic bacterium Clostridium beijerinckii and the thermophilic bacterium Thermoanaerobium brockii: preparation, characterization and molecular symmetry. Acta Crystallographica. D 52 (1996), pp 882-886.

S.W. Cowan-Jacob, J. Rahuel, A. Nagai, G. Iwasaki, D. Ohta. Crystallization and preliminary crystallogrphic analysis of cabbage histidinol dehydrogenase. Acta Crystallographica. D 52 (1996), 6, pp 1188-1190.

E. Conti, L.F. Lloyd, J. Atkins, N.P. Franks, P. Brick. Crystallization and preliminary diffraction studies of firefly luciferase from Photinus pyralis. Acta Crystallographica. D 52 (1996), 4, pp 876-878.

N.E. Chayen, E.J. Gordon, S.E.V. Phillips, E.E.G. Saridakis, P.F. Zagalsky. Crystallization and initial x-ray analysis of beta-crustacyanin, the dimer of apoproteins A(2) and C-1, each with a bound astaxanthin molecule. Acta Crystallographica. D 52 (1996), pp 409-410.

B.P. O’Hara, A.M. Hemmings, D.J. Buttle, L.H. Pearl. Crystal-structure of glycyl endopeptidase from carcia-papaya - a cysteine endopeptidase of unusual substrate-specificity. Biochemistry. 34 (1995), 40, 13190-13195.

R. Savva, L.H. Pearl. Cloning and expression of the uracil-DNA glycosylase inhibitor (UGI) from bacteriophage-PBS-1 and crystallization of a uracil-DNA glycosylase-UGI complex. Proteins-Structure Function and Genetics. 22 (1995), 3, pp 287-289.

L.F. Lloyd, P. Brick, L. Mei-Zhen, N.E. Chayen, D.M.Blow. Many crystal forms of human immunodeficiency virus reverse transcriptase. Journal of Molecular Biology. 217 (1991), pp 19-22.

S.A. Wilson, N.E. Chayen, A.M. Hemmings, R.E. Drew, L.H. Pearl. Crystallization of and Preliminary X-ray Data for the Negative Regulator (AmiC) of the Amidase Operon of Pseudomonas-aeruginosa. Journal of Molecular Biology. 222 (1991), 4, pp 869-871.

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