•Amount of Protein
–Hanging drop, sitting drop, and capillary
techniques use copious amounts of protein and crystallizing agent
–Microbatch diffusion uses 300nl of protein
per condition (in this experiment)
–Microbatch allows use of same mother liquor
for many experiments
•
•Time
–Hanging drop, sitting drop, and capillary methods
require a great deal
of time because solutions must be degassed
–Douglas
robots only need initial set up and then the robot completes the experiment