Crystallization of Oxygen Sensitive Protein
•We have purified and crystallized four proteins that are not stable in an oxygen environment
•The structure of one of these proteins (rubrerythrin) has been solved using anaerobic techniques as of this moment.
–This protein was previously solved with zinc being coordinated in an aerobic environment
–The native protein contains iron but is unstable in oxygen
–We now have the native iron form of this oxygen labile protein through using this system