Crystallization of Oxygen Sensitive
Protein
• We have purified and crystallized four proteins that are
not stable in an oxygen environment
• The structure of one of these proteins (rubrerythrin) has
been solved using anaerobic techniques as of this
moment.
– This protein was previously solved with zinc being coordinated in
an aerobic environment
– The native protein contains iron but is unstable in oxygen
– We now have the native iron form of this oxygen labile protein
through using this system