A novel microseeding method for the crystallization of membrane proteins in lipidic cubic phase.
Acta F, 2016.
Kolek, S. A., Braeuning, B., & Shaw Stewart, P. D
Automation in biological crystallization. Acta F, 2014.
Shaw Stewart, P., & Mueller-Dieckmann, J.
Membrane protein structure determination—the next generation, 2014.
Moraes, I., Evans, G., Sanchez-Weatherby, J., Newstead, S., & Stewart, P. D. S.
Structure of arylamine N-acetyltransferase from Mycobacterium
tuberculosis determined by cross-seeding with the homologous protein from M. marinum: triumph over adversity. Acta D, 2013.
Abuhammad, A., Lowe, E. D., McDonough, M. A., Shaw Stewart, P. D., Kolek, S. A.
, Sim, E., & Garman, E. F.
'Random Microseeding: A Theoretical and
Practical Exploration of Seed Stability and Seeding Techniques for Successful
Protein Crystallization'. Cryst. Growth Des., 2011, 11
(8), pp 3432–3441.
Patrick D. Shaw Stewart, Stefan A. Kolek, Richard A. Briggs,
Naomi E. Chayen and Peter F.M. Baldock. On-line at
http://pubs.acs.org/doi/abs/10.1021/cg2001442. If you do not
have a subscription to Crystal Growth and Design, click
here to obtain a free copy.
Full list of references, updated January 2016
A list of publications describing Oryx, theoretical studies using Oryx and
This list includes publications by Douglas Instruments
Microseed Matrix Screening with Lipidic Cubic Phase, June 2016
Microbatch-under-oil for XFEL, January 2016
Advanced Microseeding including LCP, December 2015
Robotic LCP dispensing, July 2015
Microseed it!, October 2014
Coupling counterdiffusion and micro-seeding techniques to increase
protein crystallization succeed, ICCBM 13
Articles and Research Reports
Further data mining of the PDB includes the concentration of protein, the
temperature, and the pH used in crystallization experiments.
Research report 10, September 2011
Microseed Matrix Screening (MMS).
Research report 9, August 2007
Data mining of the PDB reveals the most successful organic and salt
Research report 8, August 2006
Screening: a summary of studies comparing microbatch with vapor
Research report 7, June 2003
Membrane proteins crystallized using microbatch. We know of four so far.
Research report 6, March 2001
Analysis of open reading frames from Pyrococcus furiosus
Research report 5, June 1999
Practical Experimental Design Techniques for Automatic and Manual
(published in the Journal of Crystal Growth 196 (1999) 665 - 673)
Research report 4, August 1998
A Comparison of Microbatch and Vapor Diffusion for Initial Screening of Crystallization
Conditions (published in the Journal of Crystal Growth 168 (1996) 170-174)
Research report 3, November 1995
Harvesting Crystals from Microbatch for Cryocrystallography
Research report 2, October 1995
Crystallization of a protein by microseeding after establishing its phase
Research report 1, August 1995