Crystallization tips, techniques and experiments:

MMS Microseeding

Information about random Matrix Microseeding (rMMS)

  • Introduction to rMMS
  • Instructions for making Seed Stock
  • Making Seed Stock from MB under oil
  • Help with rMMS - online questionnaire
  • Microbatch Under Oil

    Under Oil Crystallization

  • Introduction to Microbatch Under Oil
  • Microbatch Experiments with Oryx8
  • Plates for Under Oil Crystallization
  • Harvesting from isopropanol Under Oil
  • 'Cross Matrix' Optimization

    Systematically test seed stock dilution, additive ingredients or multiple protein samples

  • 'Cross Matrix' Optimization with Oryx 8
  • Microseed dilution optimization
  • 'Reshuffling' optimization
  • Lipidic Cubic Phase (LCP)

    Information about LCP dispensing with Oryx

  • Preparing LCP with our mixer
  • LCP screening instructions
  • rMMS with LCP: method in Acta F
  • Scaling Up

    A few simple guidelines for scaling up from nano-drops to micro-drops

  • Scaling Up
  • Vapor Diffusion to Microbatch

    Three simple rules of thumb allow users to convert vapor diffusion crystallization conditions to microbatch

  • VD to MB: three simple rules
  • Calculator for VD-MB conversion
  • Optimization

    Optimization Methods with Oryx

  • Rational experimental design techniques
  • Optimization with Oryx8
  • Optimization for XFEL poster
  • Instructions: Optimization with Oryx8
  • 24 Well Hanging Drop

    Dispensing to 24 well Cover Slides with Oryx

  • 24-Well Hanging drop: Oryx4 and 8
  • Hanging drop experiments
  • Crystallization Plate Tips

    Tips and tricks for crystallization plates

  • Cross-polarization with sitting drop plates
  • Cards for Oryx users

    Simple step-by-step instructions for users covering maintenance and experiments

  • Cards for Oryx users
  • Other Links

  • Consumable supplies
  • DLS for Cryo-EM

  • DLS screening for Cryo-EM
  • DIXC screen
  • The 'Good Dispensing Guide'

  • The 'Good Dispensing Guide'
  • Oryx specifications

  • Oryx8 and Oryx4 specifications
  • OryxNano specifications
  • MCC controller specifications
  • Information for new users



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