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See also:

  • New user information and software requirements
  • Oryx8 and Oryx4 specifications
  • OryxNano specifications
  • MCC controller specifications
  • March 2018

    Dear New Oryx user,

    Thank you for ordering an Oryx protein crystallization system. Before we visit to install the system, it would be helpful if you could equip yourselves with the following items:

    PC with Windows 10, 8 or 7. Monitor or laptop screen with resolution of at least 1280 x 960, at least 1 GB RAM, CD ROM drive, and spare USB port.

    About 1.5 meters of bench space. (It is helpful if the bench is quite deep, around 80 cms, although this is not essential.)

    Spare mains electricity socket: 1 socket required for the Oryx system. (extra socket(s) required for control PC and monitor.)

    Protein load strips from ThermoFisher Scientific : "Strips of 8 Low Profile Tubes" catalogue # AB-0771 (Natural). We will supply 240 strips with the machine. Though these look like ordinary PCR strips, they have a particularly low profile which allows the Oryx to reach the bottom to load all protein provided.

    A 12-channel pipette for filling reservoirs, such as Transferpette-12 (20-200l) by Brand, available from www.brand.de. Alternatively, for faster pipetting we recommend the Liquidator96. This 96-channel manual pipetter will dispense to 96 reservoirs in one go.

    For microbatch-under-oil experiments you will need:

    Light paraffin oil, silicon oil and Als oil (available from Hampton Research.)

    Regular 96-well microlitre plates (around 300 microlitres per well) such as polypropylene 96-well source plates by SigmaAldrich (catalogue # M9685 www.sigmaaldrich.com.)

    For LCP experiments you will need:

    Lipid such as monoolein for preparing LCP.

    Regular 96-well microlitre plates (around 300 microlitres per well) such as polypropylene 96-well source plates by SigmaAldrich (catalogue # M9685 www.sigmaaldrich.com.)

    For Oryx8 optimization experiments:

    A printer or network connection to print XStep experiment designs.

    Please prepare some proteins and screening solutions so that we can try out the system during the installation.  The ideal format for the screening solutions is a deep-well plate (e.g. Thermofisher catalogue # AB-0932.)

    For Oryx8, please also prepare some proteins and buffers etc. for optimization.  Note that the protein, precipitants, buffers etc should be as concentrated as possible.  (If possible, the protein concentration should be twice that used in vapor diffusion, and the precipitants should be saturated.)

    We look forward to installing your system.

    Best regards,

    Douglas Instruments

     

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