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Douglas Instruments News


May 2016

Douglas Instruments has developed a method of further scaling up Lipidic Cubic Phase (LCP) experiments. The method can be used to prepare larger volumes of micro crystals for use as an LCP seed stock or for serial data collection

 

 

March 2016

Douglas Instruments has published a study adapting the rMMS Microseeding method for use with Lipidic Cubic Phase (LCP): A novel microseeding method for the crystallization of membrane proteins in lipidic cubic phase. The method involves scaling up crystallization conditions and dispensing LCP containing micro crystals to a fresh screen. Our trials showed an increase in crystallization hits of almost an order of magnitude.

 

 

May 2014

Douglas Instruments has released the Lipidic Cubic Phase (LCP) dispensing option for Oryx8 and Oryx4.  The arm mounted positive displacement syringe drive can dispense LCP volumes as small as 10 nl and up to 1 l or larger. The LCP dispensing arm is designed to be simple to use - yet make use of the powerful range of experiment scripts available on Oryx systems.

 

 

October 2013

Vapor diffusion optimization is now available with Oryx8. The robot can quickly fill the reservoirs of sitting drop or source plates with essentially unlimited volumes using the air-driven 1 ml tip in combination with the 7-bore microtip. The reservoirs are filled first, and then the drops can be dispensed independently.

 

 

April 2013

Douglas Instruments has released a new experiment script for 24 well cover slide dispensing. It is now possible to dispense screening, optimization or seeding experiments to 22mm or 18mm cover slides.

 

 

October 2011

The company has released a new script for Combinatorial Optimization.  This uses a very simple idea - combining up to 8 reservoir solutions with up to 12 additives - but it has many powerful applications.  For example, it can be used to "reshuffle" the ingredients of several hits; good crystals may result when the precipitant from one hit is combined with the buffer or salt from another hit.  Such "targeted screens" often produce well-formed diffracting crystals, starting with several less-promising conditions.

 

 

May 2011

Douglas Instruments has published a major study of the use of microseeding in protein crystallization.  (To give an idea of the scale of the study, crystallization data was collected from over 30,000 drops.)  The theory of microseeding is explored with phase diagrams, and a method of predicting which solutions can be used to suspend seed crystals is described.  We also explain how the number of salt crystals can be reduced, how to use seeding with protein complexes, and how to encourage crystallization before you have found your first crystals.  The paper is available at http://pubs.acs.org/doi/abs/10.1021/cg2001442.  If you do not have a subscription to Crystal Growth and Design, click here to obtain a free copy.

 

 

March 2011

Douglas Instruments and the Laboratorio de Estudios Cristalograficos, Granada, Spain, have published an article about using microseeding with capillary counterdiffusion.  It is available at http://pubs.acs.org/doi/abs/10.1021/cg101380j

 

 

March 2011

Douglas Instruments and the other members of the OptriCryst Consortium have published an article in Crystal Growth and Design.  It describes the application of microseeding, capillary counterdiffusion, dual polarization interferometry, in situ dynamic light scattering, and crystal growth in gels to protein crystal optimization.  It is available at http://pubs.acs.org/doi/abs/10.1021/cg1013768

 

 

September 2010

Congratulations Angeles of LEC, Granada and Richard of Douglas Instruments for winning a poster prize at ICCBM, Dublin. The poster, entitled 'Coupling counterdiffusion and microseeding techniques to increase protein crystallization success', can be found at Poster-Dublin-ICCBM

 

 

December 2009

Douglas Instruments was invited to contribute an Article about microseeding to the December issue of the SER-CAT Spectrum newsletter.  For more information about SER-CAT visit http://www.ser.aps.anl.gov/.

 

 

 

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