Research

Publications

A novel microseeding method for the crystallization of membrane proteins in lipidic cubic phase. Acta F, 2016.
Kolek, S. A., Braeuning, B., & Shaw Stewart, P. D
On-line at http://scripts.iucr.org/cgi-bin/paper?ub5090

Automation in biological crystallization. Acta F, 2014.
Shaw Stewart, P., & Mueller-Dieckmann, J.
On-line at http://scripts.iucr.org/cgi-bin/paper?s2053230x14011601

Membrane protein structure determination—the next generation, 2014. BBA Biomembranes
Moraes, I., Evans, G., Sanchez-Weatherby, J., Newstead, S., & Stewart, P. D. S.
On-line at http://www.sciencedirect.com/science/article/pii/S0005273613002447

Structure of arylamine N-acetyltransferase from Mycobacterium tuberculosis determined by cross-seeding with the homologous protein from M. marinum: triumph over adversity. Acta D, 2013.
Abuhammad, A., Lowe, E. D., McDonough, M. A., Shaw Stewart, P. D., Kolek, S. A. , Sim, E., & Garman, E. F.
On-line at http://scripts.iucr.org/cgi-bin/paper_yard?wd5214

'Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization'. Cryst. Growth Des., 2011, 11 (8), pp 3432–3441.
Patrick D. Shaw Stewart, Stefan A. Kolek, Richard A. Briggs, Naomi E. Chayen and Peter F.M. Baldock. On-line at http://pubs.acs.org/doi/abs/10.1021/cg2001442.  If you do not have a subscription to Crystal Growth and Design, click here to obtain a free copy.

Full list of references, updated January 2016
A list of publications describing Oryx, theoretical studies using Oryx and New methods

This list includes publications by Douglas Instruments


Posters

Microseed Matrix Screening with Lipidic Cubic Phase, June 2016

Microbatch-under-oil for XFEL, January 2016

Advanced Microseeding including LCP, December 2015

Robotic LCP dispensing, July 2015

Microseed it!, October 2014

Coupling counterdiffusion and micro-seeding techniques to increase protein crystallization succeed, ICCBM 13


Articles and Research Reports

Further data mining of the PDB includes the concentration of protein, the temperature, and the pH used in crystallization experiments.
Research report 10, September 2011

Microseed Matrix Screening (MMS).
Research report 9, August 2007

Data mining of the PDB reveals the most successful organic and salt precipitants
Research report 8, August 2006

Screening: a summary of studies comparing microbatch with vapor diffusion.
Research report 7, June 2003

Membrane proteins crystallized using microbatch. We know of four so far.
Research report 6, March 2001

Analysis of open reading frames from Pyrococcus furiosus
Research report 5, June 1999

Practical Experimental Design Techniques for Automatic and Manual Protein Crystallization
(published in the Journal of Crystal Growth 196 (1999) 665 - 673)

Research report 4, August 1998

A Comparison of Microbatch and Vapor Diffusion for Initial Screening of Crystallization Conditions (published in the Journal of Crystal Growth 168 (1996) 170-174)
Research report 3, November 1995

Harvesting Crystals from Microbatch for Cryocrystallography
Research report 2, October 1995

Crystallization of a protein by microseeding after establishing its phase diagram
Research report 1, August 1995


 

 

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